![]() The fl uorescence has an excitation maxima at approximately 490 nm.ġ. There is no need to add it to the electrophoresis buffer or casting gel. Protocol SafeView Plus™ can be used as a substitute for ethidium bromide for post electrophoresis DNA staining. View the results under UV or blue LED light. Run gel electrophoresis with 5 μl SafeViewTM Classic / SafeViewTM FireRed per 100 ml buffer. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.ĥ. For agarose gel, let the solution cool down to 60 - 70oC and cast the gel. Mix gently the solution should have no air bubbles.Ĥ. Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.ģ. ![]() Prepare a 100 ml agarose or polyacrylamide solution.Ģ. Protocol SafeView™ Classic and SafeView™ FireRed (Protocol )ġ. Note: All Safeview™ Classic, SafeView™ FireRed, and Safeview™ Plus will work with blue light. Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).SafeView™ FireRed Detect as little as 0.3 – 0.6 ng of DNA per gel band. S afeView Plus™ Detect as little as 0.05 – 0.1 ng of DNA per gel band. ![]() ![]() ![]() SafeView™ Classic Detect as little as 0.1 – 0.3 ng of DNA per gel band. SafeView™ can be viewed under UV light or blue light View and document your results as you would with EtBr staining.
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